Protocols Memes

The real PCR protocol they don't teach you in textbooks! Three hours meticulously pipetting, carefully programming thermal cycles, and precisely following every step... only for your gel to show absolutely nothing. That beautiful moment when you realize you just wasted two days of your life because you forgot to add the primers. Or maybe your DNA degraded. Or perhaps the PCR gods simply decided today wasn't your day. The final step of sobbing dramatically on the lab floor is actually essential for proper scientific grieving. It's basically peer-reviewed at this point.